ThemechanismofenzymeactionFoodBiochemistry食品生物化学
ThemechanismofenzymeactionFoodBiochemistry食品生物化学
目录CONTENTS123ThemechanismofenzymeactionThespeedofenzymaticreactionisaffectedFactorofIsolation,purificationandactivitydeterminationofenzymes
Themechanismofenzymeaction01
Themechanismofenzymeaction6.2CatalysisandactivationenergyofenzymesThecatalyticactionofenzymesandcatalystsismainlytoreducetheactivationenergyrequiredforthereaction,sothatthesameenergycanactivatemoremolecules,therebyspeedingupthereaction.Foodbiochemistry
Themechanismofenzymeaction6.2IntermediateproducttheoryThistheoryholdsthatwhenanenzymecatalyzesachemicalreaction,theenzyme(E)combineswiththesubstrate(S)toformanunstableintermediate(ES),whichisthenbrokendownintoaproduct(P)andreleasestheenzyme.Foodbiochemistry
Themechanismofenzymeaction6.2InducedfittheoryThistheoryholdsthatwhentheenzymemoleculeisclosetothesubstrate,theenzymeisinducedbythesubstratetoundergoconformationalchangesinfavorofbindingthesubstrate,andthesubstrateconformationisalsochanged,andtheenzymeandthesubstratearecomplementaryandfittoreact.Foodbiochemistry
Themechanismofenzymeaction6.2TheactivecenteroftheenzymeTheenzymeactivecenteroractivesitereferstoaspecificregionthatbindsdirectlytothesubstrateandtransformsitintoaproduct.Theactivecenterofenzymeusuallyhasabindingsiteandacatalyticsite.Foodbiochemistry
Themechanismofenzymeaction6.2ZymogenandzymogenactivationTheprecursorofanenzymethatisnotbiologicallyactiveiscalledzymogen.Afterthespecificactionofproteolyticenzyme,theconformationchangestoformtheactivesiteoftheenzyme,sothattheenzymehasactivity,thisactivationprocessiscalledtheactivationoftheenzyme.Foodbiochemistry
Factorsaffectingtherateofenzymaticreaction02
Themechanismofenzym
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